Chikungunya Antigen ELISA
Chikungunya has recently emerged as an important arboviral infection of medical importance, with explosive outbreaks in many areas of India and Indian Ocean islands. There is no effective therapy or prophylaxis available against this disease.Therefore early diagnosis plays an important role in proper control and effective management of patients.
This assay is based on the principle of double antibody sandwich ELISA. Purified rabbit anti-Chikungunya virus polyclonal antibody was coated on the ELISA wells to capture CHIKV antigen in patient sera. After binding, the rabbit anti-CHIKV antibody was used in a sandwich format. The resulting complex is detected using enzyme substrate reaction. Appearance of color change to brown indicates positive result.
This kit is validated with more than 100 clinical samples (Serum and CSF), collected from recent Chikungunya outbreaks in different part of India (2006-2008). It revealed >80% correlation with virus isolation and gene based molecular assays. The present innovation is a kit for early detection of Chikungunya (CHIKV) with high degree of sensitivity and specificity is based on the principle of double antibody sandwich ELISA. CHIKV is an insect-borne virus, of the genus, Alphavirus, that is transmitted to humans by virus-carrying Aedes mosquitoes in a human subject of Chikungunya infection. The kit will be useful for early clinical diagnosis of Chikungunya virus. Purified rabbit anti-Chikungunya virus polyclonal antibody was coated on the ELISA wells to capture CHIKV antigen in patient sera. After binding, the rabbit anti-CHIKV antibody was used in a sandwich format. The resulting
complex is detected using enzyme substrate reaction. Appearance of color change to brown indicates positive result
These kits will be useful for early diagnosis of Chikungunya infection with high sensitivity and specificity.