Dengue IgM ELISA
Dengue is the most important arboviral infection of medical importance. It is now endemic in many parts of tropics and sub tropics, including India. There is no effective therapy or prophylaxis available against this disease. Therefore early diagnosis plays an important role in proper control and effective management of patients. The innovation is a type of medical equipment and reagents in the form of kit for early detection of dengue fever. The subject technology is based on indirect ELISA principle, using recombinant dengue protein antigen. The wells of ELISA plate is coated with recombinant dengue antigens. The Dengue specific antibodies present in the test sera bind with antigen. The reaction is further processed with the addition of antihuman IgM-HRP conjugate. The reaction is observed through colorimetric interaction with chromogen. Color development is indicative of the presence of Dengue virus specific IgM antibodies in the test sample. The technology claims that the cross reactivity with other co-circulating members of flavivirus grous are ruled out by employing recombinant antigen thereby making the kit more specific for dengue virus only. Whereas the commercially available Dengue IgM ELISA kits are reported to have false positive results with malaria, leptospira, typhoid etc This kit is validated with more than 100 serum samples collected from recent Dengue outbreaks in India. It revealed >85% correlation with widely used commercial diagnostic kit .