Chikungunya RT-LAMP
Chikungunya has recently emerged as an important arboviral infection of medical importance, with explosive outbreaks in many areas of India and Indian Ocean islands. There is no effective therapy or prophylaxis available against this disease. Therefore early diagnosis plays an important role in proper control and effective management of patients.
This assay is intended for detection of genomic viral RNA in clinical samples (serum and CSF) as well as in culture supernatant. This is a new generation innovative gene amplification assay and is based on the principle of autocyclic strand displacement mechanism employing a set of six primers comprising two outer, two inner and two loop primers that recognize eight distinct regions spanning over 205 bp conserved regions of structural E1 gene.
The result can be monitored either in the form of visual turbidity or visual fluorescence through color change by naked eye. The better appreciation of apple green fluorescence can be achieved by a simple UV hand held torch.
This assay has been extensively validated with more than 500 suspected serum and CSF samples at DRDE Gwalior, NIMS Hyderabad, CIIMS Nagpur and Kottayam Dist Hospital Kerala. The result indicated excellent correlation in terms of sensitivity and specificity compared to RT-PCR.
This technology is based on isothermal gene amplification principle for detection of genomic viral RNA in clinical samples (serum and CSF) as well as in culture supernatant. This is a new generation innovative gene amplification assay and is based on the principle of autocyclic strand displacement mechanism employing a set of six primers comprising two outer, two inner and two loop primers that recognize eight distinct regions spanning over 205 bp conserved regions of structural E1 gene. The result can be monitored either in the form of visual turbidity or visual fluorescence through color change by naked eye. The better appreciation of apple green fluorescence can be achieved by a simple UV hand held torch.